| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Wilson Memorial | A | Miller, Paige | Baska, Kira | Armstrong, Taylor | Martin, Cameron | 1:48.90 |
| 3 | Fluvanna County | A | Harris, Prophett | Martin, Colby | Hart, Shea | Payne, R'tia | 1:48.00 |
| 4 | East Rockingham High School | A | Crider, Ashley | Breeden, Chandler | Rodriguez, Glendealiz | Jones, Andrew | 1:42.72 |
| 5 | Fort Defiance | A | Chiles, Sophia | Lawson, Hunter | Jones, Jacob | Watros, Chris | 1:45.24 |
| 6 | Spotswood | A | Russell, Abby | Smith, Robby | McClure, Elizabeth | Atkins, Carter | 1:48.84 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Eastern Mennonite School | A | Ferrell, Joely | Albers, Gabriel | Litwiler, Sydney | Vrolijk, Chad | NT |
| 4 | Harrisonburg | A | Seefried, Jacob | Seefried, Layla | Vega-Garcia, Jay | Mutambayi, Joel | NT |
| 5 | Alleghany | A | Thompson, Maggie | Dobbs, Ivan | Bryant, Olivia | Reeves, Nehemiah | NT |
| 6 | Broadway | A | Ryan, Mia | Petrie, Logan | Wuenschel, Morgan | Showalter, Kyle | NT |