| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Alleghany | A | Reeves, Nehemiah | Bryant, Olivia | Dobbs, Ivan | Krabbe, Aimee | DNS |
| 2 | Wilson Memorial | A | 54.70 | ||||
| 3 | Fort Defiance | A | 51.24 | ||||
| 4 | Spotswood | A | 50.05 | ||||
| 5 | East Rockingham High School | A | Breeden, Chandler | Jones, Makayla | Brown, Darrias | Jefferson, Koschinya | 50.16 |
| 6 | Fluvanna County | A | 52.00 | ||||
| 7 | Harrisonburg | A | NT | ||||
| 8 | Eastern Mennonite School | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Thurgood Marshall Ac | A | NT |