| $ | National | 1:26.09 | Poly, L. Beach, CA | |
| # | Current US #1 | 1:28.76 | ||
| ^ | Facility | 1:27.61 | Western Branch HS | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 3 | McKinley Tech High School | A | 1:32.95 | ||||
| 4 | Battlefield | A | 1:32.70 | ||||
| 5 | Colonial Forge | A | 1:32.72 | ||||
| 6 | Hermitage | A | 1:32.94 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Western Branch High School | A | 1:32.62 | ||||
| 3 | Northeast Senior High School | A | 1:32.43 | ||||
| 4 | Fork Union | A | 1:31.66 | ||||
| 5 | L.C. Bird | A | 1:32.32 | ||||
| 6 | William Fleming | A | 1:32.07 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Blacksburg High School | A | 1:31.22 | ||||
| 2 | Bishop McNamara High School | A | 1:31.19 | ||||
| 3 | Liberty Christian Academy | A | 1:30.86 | ||||
| 4 | Dock Mennonite Academy | A | 1:31.37 | ||||
| 5 | Central High School | A | DNF | ||||
| 6 | Westlake High School | A | 1:30.78 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Henry A. Wise High School | A | 1:29.00 | ||||
| 3 | Nansemond River | A | 1:29.17 | ||||
| 4 | DeMatha Catholic High School | A | 1:30.43 | ||||
| 5 | Bullis School | A | 1:27.00 | ||||
| 6 | Potomac Senior High School | A | DNF |