| $ | National | 8:53.67 | B & G. Brooklyn, NY | |
| # | Current US #1 | 9:20.10 | ||
| ^ | Facility | 9:28.46 | West Springfield HS | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Riverbend High School | A | Browning, Aleigh | Fairbanks, Caroline | Horne, Kenady | Vaughn, Elizabeth | 10:29.98 | 
| 2 | Albemarle | A | Douvas, Julia | Heckman, Amanda | Leach, Sarah | Zarzyski, Elynore | 10:30.00 | 
| 3 | Stafford High School | A | Calvert, Katherine | Jones, Caroline | Lambert, Greta | Lambert, Maria | DQ | 
| 4 | Harrisonburg | A | Amine, Yodit | Campillo, Abby | Deloney, Sarah | Miller, Hannah | 10:38.93 | 
| 5 | Louisa County High School | A | Aaron, Erica | Caicedo, Cierra | Campbell, Alayna | Madison, Sydney | 10:40.41 | 
| 6 | James River (Midlothian) | A | Covington, Megan | Galdamez, Jenny | Gregory, Allison | Mullis, Linda May | 10:57.09 | 
| 7 | McKinley Tech High School | A | Garcia, Maria | Johnson, Miasia | Lenhardt, Camice | Tesfai, Ruth | 11:03.02 | 
| 8 | Wilson Memorial | A | Baucom, Paige | Davis, Ashtyn | Farley, Olivia | Sanders, Camille | 11:12.36 | 
| 9 | Rocky River High School | A | Alexander, Nadia | Davis, Torri | Schofield, Amayah | Sidbury, Nyah | DNS | 
| 10 | Liberty Christian Academy | A | Kearney, Heidi | Miller, Lauren | Soistmann, Elle | Weigner, Meghan | 11:15.87 | 
| 11 | James River (Midlothian) | B | Cross, Catherine | Galdamez, Jenny | Gregory, Allison | Jamison, Madalyn | DNS | 
| 12 | Lord Botetourt | A | Hernandez, Sofia | Hornback, Brooklyn | Jackson, Brielle | Seibel, Claire | 12:54.30 | 
| 13 | Bishop McNamara High School | A | Dantzler, Jasmine | Hill, Jalyn | Nichols, Tamra | Ramsey, A'Nya | DNS | 
| 14 | King George | A | Bailey, Ellianna | Desque, Molly | Koepfinger, Hannah | Wingeart, Aubrey | NT | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Damascus High School | A | Ancalmo, Juliana | Delaplaine, Heather | Kameka, Melissa | Kameka, Samantha | 9:37.16 | 
| 2 | Western Albemarle | A | Berg, Julia | Clay, Zoe | McGahren, Caroline | Santoro-Adajian, Alyssa | 9:48.24 | 
| 3 | Green Hope | A | Eastwood, Lindsey | Fowler, Bailey | Parks, Jordyn | Rodriguez, Alessandra | DNS | 
| 4 | Ocean Lakes | A | Bowers, Savannah | King, Meredith | Kubicki, Natalie | Nery, Lauren | 9:59.24 | 
| 5 | Saint John Paul the Great Catholic High School | A | Conner, Sammi | Dunlop, Annie | Garster, Claire | Kreitzer, Rachel | 10:05.00 | 
| 6 | Massaponax | A | Bautista, Rebekah | Boggan, Alexa | Hudson, Julianne | Jean-Bart, Hillary | 10:15.00 | 
| 7 | Winston Churchill High School | A | Bush, Hannah | Iyer, Anjali | Myers, Perrin | Primich, Alexandria | 10:16.51 | 
| 8 | Grimsley | A | Bathgate, Lorien | Kincaid, Emma | Neese, Rhodes | Oberlies, Jessica | 10:20.00 | 
| 9 | Colonial Forge | A | Bignell, Caroline | Fick, Allison | Kiel, Brenna | Savoie, Maegan | 10:23.51 | 
| 10 | Deep Run | A | Brooks, Eva | Duimstra*, Sydney | Schindel, Riley | Snow*, Lily | 10:25.00 | 
| 11 | William Byrd High School | A | Bond, Kayland | Burek, Julia | Fletchall, Morgan | Tardy, Brynn | 10:25.39 | 
| 12 | James Monroe | A | Beringer, Ginny | Cobey, Lila | Kerr, Eva | Stinchcomb, Carrie | DNS | 
| 13 | St. Stephen's & St. Agnes School | A | Androus, Audrey | Burnett, Caroline | Carr, Charlotte | Cummings, Nyia | 10:28.23 |