| $ | National | 2:40.72 | Samantha Watson | Rush-Henrietta, NY | 
| # | Current US #1 | 2:53.55 | ||
| ^ | Facility | 2:47.93 | Ericka Charles | Unattached | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Wanderer, Samantha | SR | Grafton High School | 3:05.06 | 
| 2 | Cantrell, Jessica | JR | Broadway | 3:06.23 | 
| 3 | Kenney, Allison | JR | Grafton High School | 3:07.03 | 
| 4 | Rodriguez, Alessandra | JR | Green Hope | 3:07.53 | 
| 5 | Berg, Julia | JR | Western Albemarle | 3:07.72 | 
| 6 | Caden, Maya | JR | Grafton High School | 3:08.59 | 
| 7 | Hager, Rachel | JR | Glen Allen High School | 3:09.23 | 
| 8 | Parlier, Calli | SR | Apex | 3:09.30 | 
| 9 | Daley, Alexa | JR | Grafton High School | 3:09.96 | 
| 10 | Hanson, Kaleigh | JR | Croatan | 3:10.00 | 
| 11 | Henneberger *, Ashley | SR | Glen Allen High School | 3:10.17 | 
| 12 | Taranta, Nadia | SR | Friends' Central | 3:10.50 | 
| 13 | Ammons, Lauren | SR | Sanderson | 3:10.91 | 
| 14 | Fowler, Bailey | JR | Green Hope | 3:11.32 | 
| 15 | Gardner, MacKenzie | JR | Tabb | 3:11.83 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Bond, Kayland | SR | William Byrd High School | 3:12.10 | 
| 2 | Mudd, Rachel | 9 | Atlee | 3:12.25 | 
| 3 | Rudd, Riley | JR | Glen Allen High School | 3:12.41 | 
| 4 | Sudol, Emma | JR | Atlee | 3:12.81 | 
| 5 | Villanueva, Jasmine | SR | Potomac Senior High School | 3:13.19 | 
| 6 | Savoie, Maegan | JR | Colonial Forge | 3:13.35 | 
| 7 | Dunlop, Annie | JR | Saint John Paul the Great Catholic High School | 3:13.51 | 
| 8 | Tysiac, Ashley | JR | Leesville Road | 3:13.54 | 
| 9 | Bolen, Caroline | JR | Loudoun Valley | 3:13.88 | 
| 10 | Foster, Tea' | SR | Nansemond River | 3:14.14 | 
| 11 | Browning, Aleigh | JR | Riverbend High School | 3:14.36 | 
| 12 | Bir, Grace E | SO | George C. Marshall | 3:14.81 | 
| 13 | Birge, Madison | 9 | Patriot High School | 3:15.00 | 
| 14 | Kosco, Kayla | SO | Massaponax | 3:15.00 | 
| 15 | Shore, Madisun | SR | Forbush | 3:15.17 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Walker, Paityn | 9 | Colonial Forge | 3:15.40 | 
| 2 | Southall, Leigha | SR | Jefferson Forest | 3:15.67 | 
| 3 | Snyder, Leah | SO | Loudoun Valley | 3:16.00 | 
| 4 | Epstein, Elizabeth | 9 | George C. Marshall | 3:16.01 | 
| 5 | Fitch, Emily | JR | Blacksburg High School | 3:16.01 | 
| 6 | Santoro-Adajian, Alyssa | JR | Western Albemarle | 3:16.02 | 
| 7 | Polk, Allison | SR | Jefferson Forest | 3:16.20 | 
| 8 | Beegle, Olivia | JR | Floyd County | 3:16.37 | 
| 9 | Wong, Sofia | SO | Grimsley | 3:16.40 | 
| 10 | Rogers, Chloe | SO | Hidden Valley High School | 3:16.45 | 
| 11 | Ames, Julia | JR | Loudoun Valley | 3:16.73 | 
| 12 | Ratcliffe, Kate | 9 | Western Albemarle | 3:16.82 | 
| 13 | Jenkins, Riley | SR | Westfield | 3:17.21 | 
| 14 | Lass, Alyssa | SO | Gloucester High School | 3:17.24 | 
| 15 | McGahren, Caroline | JR | Western Albemarle | 3:17.66 | 
| 16 | Covington, Megan | SO | James River (Midlothian) | 3:17.88 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Jean-Bart, Hillary | SO | Massaponax | 3:18.00 | 
| 2 | Yentz, Hailey | 9 | Patriot High School | 3:18.00 | 
| 3 | Miles, Libby | JR | Poquoson | 3:18.28 | 
| 4 | Wienke, Erin | SO | William Byrd High School | 3:18.60 | 
| 5 | Taylor, Ella | 9 | Western Albemarle | 3:18.82 | 
| 6 | Rogers, Macie | SR | Patrick County | 3:18.82 | 
| 7 | Franklin*, Cabell | SO | Atlee | 3:19.07 | 
| 8 | Pollard*, Betsy | JR | Western Branch High School | 3:19.11 | 
| 9 | Dominy, Caitlin | JR | Auburn | 3:20.47 | 
| 10 | Comeskey, Hannah | JR | Leesville Road | 3:20.78 | 
| 11 | Handel, Sarah | 9 | E.C. Glass | 3:20.84 | 
| 12 | McGrady, Miranda | SR | Radford High School | 3:20.94 | 
| 13 | Deans, Tynai | SR | Woodrow Wilson | 3:22.27 | 
| 14 | Leul, Yanet | JR | Elizabeth Seton High School | 3:23.02 | 
| 15 | Hall, Grace | JR | Auburn | 3:25.00 | 
| 16 | Jean-Baptiste, Jasanyh | Western Branch High School | 3:25.00 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | O'Brien, Kate | SR | Virginia Episcopal School | 3:25.00 | 
| 2 | Beringer, Ginny | 9 | James Monroe | NT | 
| 3 | Lattimer, Julia | JR | Blacksburg High School | 3:25.39 | 
| 4 | Wilson, Katelyn | SR | Louisa County High School | 3:25.45 | 
| 5 | Musselman, Jessica | JR | Auburn | 3:26.20 | 
| 6 | Whitaker, Maya | JR | Southwest Guilford | 3:26.30 | 
| 7 | Caicedo, Cierra | JR | Louisa County High School | 3:26.36 | 
| 8 | Conrod, Abby | 8 | Nansemond-Suffolk Academy | 3:26.47 | 
| 9 | Kirtner, Ashlyn | 9 | Pulaski County | 3:26.70 | 
| 10 | Fletchall, Morgan | JR | William Byrd High School | 3:26.97 | 
| 11 | Laneve, Ashley | SO | Jefferson Forest | 3:27.24 | 
| 12 | Aronson, Malaina | SO | Leesville Road | 3:28.00 | 
| 13 | White, Julianna | SR | Sanderson | 3:28.12 | 
| 14 | Young, TataYiana | SO | Woodrow Wilson | 3:28.24 | 
| 15 | Garster, Claire | SR | Saint John Paul the Great Catholic High School | 3:28.41 | 
| 16 | Madison, Sydney | SO | Louisa County High School | 3:29.01 | 
| 17 | Mutchler, Emily | SO | Calvert High School | 3:29.75 | 
| 18 | Rice, Taylor | JR | Churchland | 3:30.24 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Burleson, Mackenzie | 9 | Cosby | 3:30.54 | 
| 2 | Novak, Emily | JR | Cosby | 3:30.54 | 
| 3 | Garner, Avery | SO | Cosby | 3:31.77 | 
| 4 | Miller, Dana | 9 | Calvert High School | 3:31.90 | 
| 5 | Hernandez, Sofia | 9 | Lord Botetourt | 3:32.00 | 
| 6 | Borter, Allison | SR | Sanderson | 3:32.11 | 
| 7 | Douglas, Brittney | JR | Calvert High School | 3:32.45 | 
| 8 | Seibel, Claire | SR | Lord Botetourt | 3:32.69 | 
| 9 | Dickerson, Mia | SR | Manchester High School | 3:33.07 | 
| 10 | Wiley, TaMira | JR | Bishop McNamara High School | 3:34.37 | 
| 11 | Walker, Mackenzie | 9 | Northside High School | 3:34.82 | 
| 12 | Brooks, Eva | SO | Deep Run | 3:35.00 | 
| 13 | Scott, Emma | JR | William Campbell Combined School | 3:35.38 | 
| 14 | Valentin, Olivia | JR | Atlee | 3:36.88 | 
| 15 | Burek, Julia | SR | William Byrd High School | 3:39.80 | 
| 16 | Nichols, Tamra | 9 | Bishop McNamara High School | 3:39.98 | 
| 17 | Canning*, Aideen | SR | Deep Run | 3:40.00 | 
| 18 | Carroll, Krystal | JR | Liberty (Bedford) | 3:40.49 | 
| 19 | Stewart, Hannah | 8 | James River (Midlothian) | 3:41.00 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Warren, Virginia | 8 | James River (Midlothian) | 3:41.14 | 
| 2 | crawley, morgan | JR | Prince George High School | 3:41.88 | 
| 3 | Hensley, Summer | SO | Amherst County | 3:42.02 | 
| 4 | Betancourt, Aura | JR | Manchester High School | 3:43.82 | 
| 5 | Galdamez, Jenny | JR | James River (Midlothian) | 3:44.82 | 
| 6 | Snow, Julia | 9 | Deep Run | 3:45.00 | 
| 7 | Mullis, Linda May | JR | James River (Midlothian) | 3:49.00 | 
| 8 | Gregory, Allison | 9 | James River (Midlothian) | 3:49.04 | 
| 9 | Patel, Vinita | SO | Northside High School | 3:49.07 | 
| 10 | Lester, Ainsley | 9 | Hidden Valley High School | 3:49.50 | 
| 11 | Buskill, Ellie | 9 | Radford High School | 3:49.51 | 
| 12 | mentzer, emma | SO | Prince George High School | 3:49.60 | 
| 13 | Kone, Jeneba | JR | James River (Midlothian) | 3:50.76 | 
| 14 | Becker*, Arielle | JR | Deep Run | 3:52.00 | 
| 15 | Hamilton, Aminah | SR | Suitland High School | 3:53.03 | 
| 16 | Crawford, Erin | 9 | Blacksburg High School | 3:56.17 | 
| 17 | Cross, Catherine | JR | James River (Midlothian) | 3:59.64 | 
| 18 | Lin, Alice | 9 | Radford High School | 4:04.29 | 
| 19 | Udofia, Angel | 9 | Osbourn | 4:08.50 | 
| 20 | Rogers, Emma | JR | Hidden Valley High School | NT |